Part:BBa_K3602005:Design

ERG Target Cas13a

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The T7 promoter was chosen from neb.com for high yield RNA synthesis. TMPRSS2:ERG has different splice variants so this part was designed to be the most common alternative splicing variation of exons. (https://international.neb.com/protocols/2015/11/24/sgrna-synthesis-using-the-hiscribe-quick-t7-high-yield-rna-synthesis-kit-neb-e2050)

Source

The sequence is designed from the ERG mRNA sequence from the human reference genome GRCh38 via ensembl (https://www.ensembl.org/).